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RNA-SeQC

Supported Tool

Fast, efficient RNA-Seq metrics for quality control and process optimization

Description

The RSeQC module parses results generated by RNA-SeQC, (not to be confused with RSeQC, which MultiQC also supports).

RNA-SeQC is a java program which computes a series of quality control metrics for RNA-seq data.

This module shows the Spearman correlation heatmap if both Spearman and Pearson’s are found. To plot Pearson’s by default instead, add the following to your MultiQC config file:

rna_seqc:
  default_correlation: pearson

File search patterns

rna_seqc/metrics_v1:
  fn: "*metrics.tsv"
  contents: "Sample\tNote\t"
rna_seqc/metrics_v2:
  fn: "*metrics.tsv"
  contents: High Quality Ambiguous Alignment Rate
rna_seqc/coverage:
  fn_re: meanCoverageNorm_(high|medium|low)\.txt
rna_seqc/correlation:
  fn_re: corrMatrix(Pearson|Spearman)\.txt