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BISCUIT

Supported Tool

BISCUIT is a software tool suite for analyzing bisulfite-converted DNA sequencing.

Description

The BISCUIT module parses logs generated by BISCUIT and the quality control script, QC.sh, included with the BISCUIT software.

Note - as of MultiQC v1.9, the module supports only BISCUIT version v0.3.16 and onwards. If you have BISCUIT data from before this, please use MultiQC v1.8.

Insert Size Distribution

The second tab of this plot uses the config option read_count_multiplier, so if millions of reads is not useful for your data you can customise this.

See Number base (multiplier) in the documentation.

File search patterns

biscuit/align_mapq:
  fn: "*_mapq_table.txt"
  contents: BISCUITqc Mapping Quality Table
  num_lines: 3
biscuit/align_strand:
  fn: "*_strand_table.txt"
  contents: BISCUITqc Strand Table
  num_lines: 3
biscuit/align_isize:
  fn: "*_isize_table.txt"
  contents: BISCUITqc Insert Size Table
  num_lines: 3
biscuit/dup_report:
  fn: "*_dup_report.txt"
  contents: BISCUITqc Read Duplication Table
  num_lines: 3
biscuit/qc_cv:
  fn: "*_cv_table.txt"
  contents: BISCUITqc Uniformity Table
  num_lines: 3
biscuit/covdist_all_base_botgc:
  fn: "*_covdist_all_base_botgc_table.txt"
biscuit/covdist_all_base:
  fn: "*_covdist_all_base_table.txt"
biscuit/covdist_all_base_topgc:
  fn: "*_covdist_all_base_topgc_table.txt"
biscuit/covdist_q40_base_botgc:
  fn: "*_covdist_q40_base_botgc_table.txt"
biscuit/covdist_q40_base:
  fn: "*_covdist_q40_base_table.txt"
biscuit/covdist_q40_base_topgc:
  fn: "*_covdist_q40_base_topgc_table.txt"
biscuit/covdist_all_cpg_botgc:
  fn: "*_covdist_all_cpg_botgc_table.txt"
biscuit/covdist_all_cpg:
  fn: "*_covdist_all_cpg_table.txt"
biscuit/covdist_all_cpg_topgc:
  fn: "*_covdist_all_cpg_topgc_table.txt"
biscuit/covdist_q40_cpg_botgc:
  fn: "*_covdist_q40_cpg_botgc_table.txt"
biscuit/covdist_q40_cpg:
  fn: "*_covdist_q40_cpg_table.txt"
biscuit/covdist_q40_cpg_topgc:
  fn: "*_covdist_q40_cpg_topgc_table.txt"
biscuit/cpg_retention_readpos:
  fn: "*_CpGRetentionByReadPos.txt"
biscuit/cph_retention_readpos:
  fn: "*_CpHRetentionByReadPos.txt"
biscuit/base_avg_retention_rate:
  fn: "*_totalBaseConversionRate.txt"
biscuit/read_avg_retention_rate:
  fn: "*_totalReadConversionRate.txt"